The seahorse bioscience xf96 extracellular flux analyzer is an instrument that simultaneously measures two energy yielding pathways. Seahorse bioscience xf96 extracellular flux analyzer wins. Seahorse xfe24 extracellular flux analyzerbased analysis of. The first published seahorse data was reported in a 2006 nature article. Glycolysis is determined through measurements of the extracellular acidification rate ecar of the surrounding media, which is predominately from the excretion of lactic acid per unit time. The xf24 can also measure respiration of isolated mitochondria. Seahorse xfe96 extracellular flux analyzer the seahorse enables quantitation of metabolomics of live cells in real time with stimulation with up to 4 drugs. Seahorse bioscience xf96 extracellular flux analyzer product. Good data with the software makes good graphic presentations. Cardamonin inhibits breast cancer growth by repressing hif. Seahorse bioscience xfe instrument overview and intended use the seahorse bioscience xfe analyzer measures the rate of change of dissolved oxygen and ph in the media immediately surrounding living cells cultured in a microplate. Seahorse xf96 v3 ps cell culture microplates, pack of 10.
Xfe extracellular flux analyzers simultaneously interrogate the two major energy producing pathways of the cell mitochondrial respiration and glycolysis in a microplate, in realtime. Ocr and ecar rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systemslevel view of cellular metabolic function in cultured cells and exvivo samples. Scanning seahorse bioscience xf96 cell culture microplates. Box of seahorse xf96 polystyrene cell culture microplates pn 101085004. The seahorse xf24 is a revolutionary instrument for studies of cellular energy metabolism. Ocr and ecar rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systemslevel view of cellular metabolic function in. Agilent technologies seahorse bioscience in vitro technologies. Seahorse xfe96 extracellular flux analyzer dartlab. Rapid analysis of glycolytic and oxidative substrate flux. Technologies seahorse bioscience is a leader in life sciences, diagnostics, and applied chemical markets. Seahorse xf cell energy phenotype test kit receives top 10. Seahorse bioscience bought bioprocessors corp in 2009. Seahorse xf cell energy phenotype test kit receives top 10 innovation award from the scientist. The xfp extracellular flux analyzer is built on innovative and proven xf technology for live cellbased assays of energy metabolism.
The seahorse extracellular flux xf analyzer seahorse bioscience is a. Finally, ocr, glycoper and atp production rates of mitochondrial oxidative phosphorylation and glycolysis were determined and analyzed on the agilents seahorse bioscience xf96 extracellular flux analyzer agilent technologies according to the manufacturers instructions and protocols seahorse bioscience, north billerica, ma, usa. The measurement of cellular bioenergetics on live cells enables timeresolved analysis and testing of multiple conditions per assay well. Seahorse xfe96 analyzers measure ocr and ecar of live cells in a 96well plate format. Only differences between the control group and the two hyperglycemic groups obp vs ogdm and obp vs ot1dm were analyzed. The seahorse extracellular flux xf analyzer seahorse bioscience is a powerful tool for measuring glycolysis and oxidative phosphorylation through oxygen consumption simultaneously in the same cells. Xfp extracellular flux analyzer from agilent cell analysis. Live metabolic profile analysis of zebrafish embryos using. View and download seahorse xf96 installation and operation manual online. Launched in 2006, the awardwinning xf extracellular flux analyzer is the industry standard for measuring cellular bioenergetics, simultaneously measuring the two major energyproducing pathways.
The agilent cell analysis software portfolio provides easytolearn, flexible toolssolutions for designing assays, instrument operation, analyzing data, and. Add 200 l of seahorse bioscience xf96 calibrant ph 7. Seahorse xf analyzers measure oxygen consumption rate ocr and extracellular acidification rate. Based analysis of cellular respiration in caenorhabditis elegans anthony l. Compact and easy to use, the xfp analyzer is designed to perform standard xf assays, and is ideal for pairwise comparisons. The xf96 extracellular flux analyzer seahorse bioscience is a noninvasive, labelfree, highthroughput instrument that measures the metabolic activity of living cells by simultaneously monitoring mitochondrial respiration and glycolysis. Mar 11, 2019 the seahorse xf cell energy phenotype test in conjunction with a seahorse bioscience xf96 extracellular flux analyzer measures the mitochondrial respiration and glycolysis under baseline and. Xfe24 extracellular flux analyzer seahorse bioscience. The agilent seahorse extracellular flux analyzer can be used to measure the oxygen consumption rate ocr and extra cellular acidification rate ecar, from which mitochondrial bioenergetics measurements can be determined including basal respiration, respiration due to atp turnover, uncoupled respirationproton leak, and maximum respiration. Software download for seahorse xf96 analyzer agilent. The oxygen consumption rate ocr and the extracellular acidification rate ecar were measured with a xf96 extracellular flux analyzer seahorse bioscience, agilent technologies.
The seahorse xf cell energy phenotype test in conjunction with a seahorse bioscience xf96 extracellular flux analyzer measures the mitochondrial respiration and. Changes in the extracellular media are caused by the consumption or production of analytes by the cells. Turn on instrument and start xf software to allow instrument to stabilize at 37 c. Nicholas school of the environment, duke university, durham, north carolina. The extracellular acidification rate ecar of niclosamide treated skov3 and ho8910 cells under the indicated conditions were measured in real time using the seahorse xf96 extracellular flux analyzer. Seahorse xf96 installation and operation manual pdf. Seahorse xfp extracellular flux analyzer the role of metabolism in cellular and physiological processes from cardiovascular function to immune response is well established, with many diseases now linked to metabolic dysfunction or reprogramming. Advances in the quantification of mitochondrial function. Seahorse xfe24 extracellular flux analyzerbased analysis. Seahorse bioscience introduces xfe the worlds most advanced.
Rapid analysis of glycolytic and oxidative substrate flux of. The company provides laboratories worldwide with instruments, services, consumables, applications, and expertise. Seahorse assay kits for example, xf cell mito stress test. Seahorse xf24 extracellular flux analyzer the seahorse xf24 is a revolutionary instrument for studies of cellular energy metabolism. Quick overview preowned and in working condition computer included with software flux analyzer part no 100900101 pre station part no 101200100 manual. The new seahorse xfp analyser has been built on the basis of the proven. The seahorse bioscience xf96 system includes xf96 analyzer. Its novel 8well miniplate format is ideal for use in pairwise comparison assays, as well as for use with primary cells and other precious samples.
This instrument measures oxygen consumption rate ocr and extracellular acidification rate ecar of living cells simultaneously in a 24 well format. Seahorse bioscience introduced extracellular flux technology to the life sciences market in 2006. High throughput screening with viaflo 96 xf96 extracellular. Integra has published a video demonstrating how its viaflo 96 handheld electronic pipette, used in conjunction with the seahorse bioscience xf96 extracellular flux analyzer, provides high throughput screening of the cellular bioenergetics of multiple cell lines. The measurement of cellular bioenergetics, the processes by which cells produce and consume energy, is fundamental to determining. Xf instruments measure cellular bioenergies in real time, in a microplate, and are used for scientific research, as well as drug discovery and development. High throughput screening of cellular bioenergetics integra. Lowdose 2deoxyglucose and metformin synergically inhibit. Seahorse bioscience xfe instrument overview and intended use the seahorse bioscience xfe analyzer measures the rate of change of dissolved oxygen and ph in the media immediately surrounding living cells cultured in a. Here, using the seahorse xf e 24 extracellular flux analyzer seahorse bioscience, massachusetts, usa we describe how to measure the fundamental parameters of the electron transport chain etc. Luz nicholas school of the environment, duke university, durham, north carolina. Ocr and ecar measurements were performed using the xf24 or xf96 extracellular flux analyzer seahorse bioscience, north billerica, ma as described. The xf96 extracellular flux analyzer was developed to provide higher throughput analysis for customers desiring to study mitochondrial function and dysfunction, obesity, diabetes, cancer, ageing disorders, cardiac disease and drug toxicity.
Measuring energy metabolism in cultured cells, including human pluripotent stem cells and differentiated cells. The agilent cell analysis software portfolio provides easytolearn, flexible tools solutions for designing assays, instrument operation, analyzing data, and. Extracellular flux analysis to monitor glycolytic rates and. The xfe24 flux analyzer measures real time kinetic of the media flux in live cellular environment to determine in vitro oxygen consumption rate ocr, and extracellular acidification rate ecar, in order to assess cellular metabolic functions such as oxidative phosphorylation, glycolysis, and fatty acid oxidation. For analyzing hpscs, an appropriate study protocol is to set the time to mix for 2 min, wait for 2 min and measure for 4 min. This fast and sensitive measurement of cellular bioenergetics is label free, enabling timeresolved analysis and. Extracellular flux an overview sciencedirect topics. The seahorse xfp analyzer is a compact and easytouse bench top instrument for analyzing energy metabolism in live cells. Seahorse xf analyzers measure oxygen consumption rate ocr and extracellular acidification rate ecar of live cells in a multiwell plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. Seahorse bioscience xf96 extracellular flux analyzer w xf.
Seahorse bioscience xf96 extracellular flux analyzer product details the xf96 extracellular flux analyzer is a fullyintegrated instrument that simultaneously measures the two major energy yielding pathways aerobic respiration and glycolysis in a convenient, microplate format. Seahorse xf24 extracellular flux analyzer department of. Briefly, cells were plated into xf24 v7 or xf96 v3 polystyrene cell culture plates seahorse bioscience, north billerica. Livecell metabolic assay platform for life science research.
The extracellular flux xf assay described here is a platebased method in which galactoseconditioned hepg2 cells were acutely exposed. Seahorse xfe96 analyzer measures ocr and ecar of live cells in a 96well plate. Accurate xf24 measurements require the analyzed cells to be grown in a. The acute extracellular flux xf assay to assess compound. Also notable is that the seahorse xf96 extracellular flux analyzer was cited as a 2009 top ten. Pdf the acute extracellular flux xf assay to assess.
The extracellular flux analyzer is a commonly used device for evaluating the performance of glycolysis and mitochondrial respiration in many cell types. Quick overview preowned and in working condition computer included with software flux analyzer part no 100900101 pre station part no 101200100 manual included. Measurement of mitochondrial respiration and glycolysis allows energetic phenotyping of cells, in order to assess functional changes. Seahorse bioscience xfe instrument overview and intended use the seahorse bioscience xfe analyzer measures the rate of change of dissolved oxygen and ph in the media immediately surrounding living cells cultured in. Wave software contains several default assay template files for the standardized agilent. Seahorse xf96 manuals manuals and user guides for seahorse xf96. Seahorse technology is based on measuring the rate of extracellular fluxes of. Agilent technologies seahorse bioscience technologies seahorse bioscience is a leader in life sciences, diagnostics, and applied chemical markets. We have 1 seahorse xf96 manual available for free pdf download. Analysis of lymphocyte mitochondrial function has been facilitated by introduction of 96well format extracellular flux xf96 analyzers, but the technology remains imperfect for analysis of human lymphocytes. Extracellular flux analyzer and prep station brand. Extracellular flux analysis to monitor glycolytic rates.
Statistical analyses were performed using sas software, version 9. The xf96 and xfe96 extracellular flux analyzer are 96 well tissue culture microplate systems allowing for a larger number of assays. View online or download seahorse xf96 installation and operation manual. Seahorse bioscience introduces xfe the worlds most. Seahorse xfe analyzers from agilent cell analysis selectscience. The xf96well microplates were analyzed using the seahorse bioscience xfe96 extracellular flux analyzer. The seahorse bioscience xf96 extracellular flux analyzer figure 1 offers the advantage of significantly increased sensitivity, enabling the detection of defects in the oxphos system in human. Released in late 2006, the xf24 analyzer was and continues to be entirely unique to the biomedical research field. High throughput screening of cellular bioenergetics. Basal ecar were measured at three time points, followed by sequential injections of the glucose 10 mm, oligomycin 1. This video demonstrates how the viaflo 96 handheld electronic pipette, used in conjunction with the seahorse bioscience xf96 extracellular flux analyzer, provides high throughput screening of the cellular bioenergetics of multiple cell lines. The company provides laboratories worldwide with instruments, services, consumables, applications, and expertise, enabling customers to gain the insights they seek. Advances in the quantification of mitochondrial function in. The extracellular flux xf assay described here is a platebased method in which galactoseconditioned hepg2 cells were acutely exposed to test compounds, then realtime changes in the oxygen consumption rate and extracellular acidification rate were simultaneously measured using a seahorse bioscience xf 96 analyzer.
Seahorse bioscience xf96 extracellular flux analyzer. This system has been used to study immune cells in a few published reports, yet a comprehensive protocol optimized particularly for lymphocytes is lacking. Seahorse bioscience announces the xf96 extracellular flux. Novel technology opens the door to the understanding of.
Easily create assay protocols and analyze data with seahorse wave software. The seahorse bioscience xf e extracellular flux analyzer measures the two major energy pathways of the cell mitochondrial respiration and glycolysis in a microplate, in realtime. Measuring energy metabolism in cultured cells, including. Seahorse xf96 installation and operation manual pdf download. The seahorse extracellular flux xfe analysers provide a convenient. Fetal hyperglycemia changes human preadipocyte function in. Furthermore, changes in mitochondrial function may regulate lymphocyte function in inflammatory diseases like type 2 diabetes. Based analysis of cellular respiration in caenorhabditis elegans. Seahorse wave desktop software is the assay design and data analysis software. Xfe24 assays apply to all fields of biomedical research.
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